Wavelet based segmentation of hyperspectral colon tissue imagery

Segmentation is an early stage for the automated classification of tissue cells between normal and malignant types. We present an algorithm for unsupervised segmentation of images of hyperspectral human colon tissue cells into their constituent parts by exploiting the spatial relationship between these constituent parts. This is done by employing a modification of the conventional wavelet based texture analysis, on the projection of hyperspectral image data in the first principal component direction. Results show that our algorithm is comparable to other more computationally intensive methods which exploit the spectral characteristics of the hyperspectral imagery data

Hyperspectral colon tissue cell classification

A novel algorithm to discriminate between normal and malignant tissue cells of the human colon is presented. The microscopic level images of human colon tissue cells were acquired using hyperspectral imaging technology at contiguous wavelength intervals of visible light. While hyperspectral imagery data provides a wealth of information, its large size normally means high computational processing complexity. Several methods exist to avoid the so-called curse of dimensionality and hence reduce the computational complexity. In this study, we experimented with Principal Component Analysis (PCA) and two modifications of Independent Component Analysis (ICA). In the first stage of the algorithm, the extracted components are used to separate four constituent parts of the colon tissue: nuclei, cytoplasm, lamina propria, and lumen. The segmentation is performed in an unsupervised fashion using the nearest centroid clustering algorithm. The segmented image is further used, in the second stage of the classification algorithm, to exploit the spatial relationship between the labeled constituent parts. Experimental results using supervised Support Vector Machines (SVM) classification based on multiscale morphological features reveal the discrimination between normal and malignant tissue cells with a reasonable degree of accuracy

Feature detection from echocardiography images using local phase information

Ultrasound images are characterized by their special speckle appearance, low contrast, and low signal-to-noise ratio. It is always challenging to extract important clinical information from these images. An important step before formal analysis is to transform the image to significant features of interest. Intensity based methods do not perform particularly well on ultrasound images. However, it has been previously shown that these images respond well to local phase-based methods which are theoretically intensity-invariant and thus suitable for ultrasound images. We extend the previous local phase-based method to detect features using the local phase computed from monogenic signal which is an isotropic extension of the analytic signal. We apply our method of multiscale feature-asymmetry measurement and local phase-gradient computation to cardiac ultrasound (echocardiography) images for the detection of endocardial, epicardial and myocardial centerline

Functional connectivity alterations in epilepsy from resting-state functional MRI

The study of functional brain connectivity alterations induced by neurological disorders and their analysis from resting state functional Magnetic Resonance Imaging (rfMRI) is generally considered to be a challenging task. The main challenge lies in determining and interpreting the large-scale connectivity of brain regions when studying neurological disorders such as epilepsy. We tackle this challenging task by studying the cortical region connectivity using a novel approach for clustering the rfMRI time series signals and by identifying discriminant functional connections using a novel difference statistic measure. The proposed approach is then used in conjunction with the difference statistic to conduct automatic classification experiments for epileptic and healthy subjects using the rfMRI data. Our results show that the proposed difference statistic measure has the potential to extract promising discriminant neuroimaging markers. The extracted neuroimaging markers yield 93.08% classification accuracy on unseen data as compared to 80.20% accuracy on the same dataset by a recent state-of-the-art algorithm. The results demonstrate that for epilepsy the proposed approach confirms known functional connectivity alterations between cortical regions, reveals some new connectivity alterations, suggests potential neuroimaging markers, and predicts epilepsy with high accuracy from rfMRI scans.Scopu

Handcrafted histological transformer (H2T):unsupervised representation of whole slide images

Diagnostic, prognostic and therapeutic decision-making of cancer in pathology clinics can now be carried out based on analysis of multi-gigapixel tissue images, also known as whole-slide images (WSIs). Recently, deep convolutional neural networks (CNNs) have been proposed to derive unsupervised WSI representations; these are attractive as they rely less on expert annotation which is cumbersome. However, a major trade-off is that higher predictive power generally comes at the cost of interpretability, posing a challenge to their clinical use where transparency in decision-making is generally expected. To address this challenge, we present a handcrafted framework based on deep CNN for constructing holistic WSI-level representations. Building on recent findings about the internal working of the Transformer in the domain of natural language processing, we break down its processes and handcraft them into a more transparent framework that we term as the Handcrafted Histological Transformer or H2T. Based on our experiments involving various datasets consisting of a total of 10,042 WSIs, the results demonstrate that H2T based holistic WSI-level representations offer competitive performance compared to recent state-of-the-art methods and can be readily utilized for various downstream analysis tasks. Finally, our results demonstrate that the H2T framework can be up to 14 times faster than the Transformer models